Proteomic and immunoassay characterization of a new food allergen from hazelnut (corylus avellana)
نویسندگان
چکیده
Methods Otherwise healthy children with oral food challenge confirmed hazelnut allergy were prospectively evaluated. Crude protein extracts were obtained from 5 hazelnut varieties, including autochthon, Northern Italy and Oregon (USA) cultivars, with phosphate saline buffer, pH 7.2. The immunoreactive protein components were identified by SDS-PAGE electrophoresis and Western immunoblotting, using patients sera as source of specific IgE. The IgE-binding protein bands were characterized by advanced proteomic strategies and tandem mass spectrometry (MS)-based de novo peptide sequencing.
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